Ribosomal protein L3



Ribosomal protein L3 (RPL3 ) is one of the 34 proteins which belong to the large 50S subunit of the prokaryotic ribosome or to the ca. 49 proteins in the large 60S subunit of the eukaryotic ribosome. It binds to 23S RRNA near the 3'-end where it nucleates the assembly of the large subunit. In the archaea like in Haloarcula marismortui the L3 is called L3P (RPL3P). The images at the left and at the right correspond to one representative RPL3, i.e. the crystal structure of Ribosomal protein L3 from Thermus thermophilus (2xg0, chain E).

3D Structures of Ribosomal protein L3
Update June 2011

L3 in 50S subunit+RNA
2xg0, 2xg2, 3kir, 3kit, 3kiw, 3kiy – TtRPL3 in 50S subunit+RNA fragments – Thermus thermophilus

2x9s, 2x9u, 3kni, 3knk, 3knm, 3kno, 2wrj, 2wrl, 2wro, 2wrr, 3hux, 3huz, 2wh2, 2wh4, 2wdi, 2wdj, 2wdl, 2wdn, 1vsp, 2v47, 2v49, 1vsa, 2hgj, 2hgq, 2hgu, 2j01, 2j03, 1yl3, 3mrz, 3ms1, 3i8f, 3i8i, 3i9c, 3i9e, 3f1f, 3f1h, 3d5b, 3d5d, 2b66, 2b9n, 2b9p, 1giy, 2y0v, 2y0x, 2y0z, 2y11, 2y13, 2y15, 2y17, 2y19, 3pyo, 3pyr, 3pyt, 3pyv - TtRPL3 in 50S subunit+5S RRNA+23S RRNA

3fin - TtRPL3 in 50S subunit+5S RRNA+23S RRNA – Cryo-EM

2wwq – EcRPL3 in 50S subunit+5S RRNA+23S RRNA+mRNA+P-site tRNA – Escherichia coli

3i1n, 3i1p, 3i1r, 3i1t, 3i20, 3i22, 3e1b, 3e1d, 3df2, 3df4, 2vhn, 2qov, 2qox, 2i2t, 2i2v, 1vs6, 1vs8, 2aw4, 2awb, 3ofd, 3og0, 1vor, 1vou, 1vow, 1voy, 1vp0, 1pnu, 1pny,3r8s, 3r8t - EcRPL3 in 50S subunit+5S RRNA+23S RRNA

3fik, 2gya, 2gyc, 3bbx, 1p85, 1p86,3izt, 3izu- EcRPL3 in 50S subunit+5S RRNA+23S RRNA – Cryo-EM

3j01 – EcRPL3 in 50S subunit+5S RRNA+23S RRNA+preprotein translocase – Cryo-EM

[3kcr]] - EcRPL3 in 50S subunit+5S RRNA+23S RRNA+preprotein translocase

2rdo - EcRPL3 in 50S subunit+5S RRNA+23S RRNA+EF-G+RRF – Cryo-EM

3bbo - RPL3 in 50S subunit+5S RRNA+23S RRNA+4.8S RRNA – Cryo-EM – spinach

3iy9 – RPL3 in 50S subunit – Leishmania taretolae

2zjq, 2zjr, 1nkw - DrRPL3 in 50S subunit+5S RRNA+23S RRNA – Deinococcus radiodurans

1kqs, 1qvg - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+CCA - Haloarcula marismortui

1jj2, 1ffk, 3cc2, 2qa4, 1s72 - HmRPL3P in 50S subunit+5S RRNA+23S RRNA

3cc7, 3cce, 3ccj, 3ccl, 3ccm, 3ccq, 3ccr, 3ccs, 3ccu, 3ccv - HmRPL3P in 50S subunit+5S RRNA+23S RRNA (mutant)

1vq4, 1vq5, 1vq7, 1vql, 1vqm, 1vqp - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+transition state analog

1vq6, 1vqk, 1vqn - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+ c-hpmn+CCA-Phe-caproic acid-biotin

1vqo - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+ CCPMN

1yhq, 1yi2, 1yij, 1yjn, 1yjw - HmRPL3P in 50S subunit+5S RRNA+23S RRNA (mutant)+antibiotic

1yj9 - HmRPL3P in 50S subunit (L22 mutant)+5S RRNA+23S RRNA

1qvf - HmRPL3P in 50S subunit+5S RRNA+23S RRNA + deacylated tRNA minihelix

1q86 - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+CCA-Phe-caproic acid-biotin

L3 in 50S subunit + antibiotics
3dll, 2zjp, 3cf5, 1xbp, 1sm1, 1nwx, 1nwy, 3pio, 3pip - DrRPL3 in 50S subunit+5S RRNA+23S RRNA+antibiotic

2ogm, 2ogn, 2ogo - DrRPL3+23S RRNA+pleuromutilin derivative

3oh5, 3oh7, 3ohj, 3ohk, 3ohz, 3oi1, 3oi3, 3oi5 – TtRPL3 in 50S subunit+5S RRNA+23S RRNA+antibiotic

2qam, 2qao, 2qoz, 2qp1, 2qba, 2qbc, 3ofc, 3ofz, 3ofq, 3ofr, 1vt2, 3orb,3oas, 3oat - EcRPL3 in 50S subunit+5S RRNA+23S RRNA+antibiotic

1k73, 1kc8, 1k8a, 1k9m, 1kd1, 1m1k, 3i55, 3i56, 3g4s, 3g6e, 3g71, 2qex, 3cpw, 3cc4, 3cd6, 2otj, 2otl, 1yit, 1q7y, 1q81, 1q82, 1n8r, 1nji, 3cxc - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+antibiotic - Halobacterium marismortui

1m90, 3cma, 3cme, 1vq8, 1vq9 - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+ sparsomycin+CCA-Phe-caproic acid-biotin

L3 in 60S subunit
3jyw - RPL3 in 60S subunit – Thermomyces lanuginosus – Cryo-EM

2ftc - RPL3 in 60S subunit+16S RRNA - bovine

1s1i - yRPL3 in 40S ribosomal subunit+18S RRNA+elongation factor 2 – Cryo-EM – yeast

3o58, 3o5h – yRPL3 in 60S subunit+25S RRNA+5SRRNA+5.8RRNA

L3 in large + small ribosomal subunit
1ml5 - EcRPL3 in 50S ribosomal subunit+30S ribosomal subunit+THX+chain release factor 2+P-site tRNA+tRNA Phe+mRNA fragment+23S RRNA+5S RRNA+16S RRNA – EM

2zkr - RPL3 in 60S ribosomal subunit+30S ribosomal subunit+28S RRNA+5.8S RRNA – Cryo-EM - dog

L3 in 50S subunit + ribosome releasing factor (RRF)
2qbe, 2qbg, 2qbi, 2qbk, 2z4l, 2z4n - EcRPL3 in 50S subunit+5S RRNA+23S RRNA+RRF

L3 in 50S subunit + various proteins
2vhm - EcRPL3 in 50S subunit+5S RRNA+23S RRNA+PDF C-terminal helix

2j28 - EcRPL3 in 50S subunit+5S RRNA+23S RRNA+signal recognition particle – Cryo-EM

1w2b - HmRPL3P in 50S subunit+5S RRNA+23S RRNA+trigger factor N-terminal